Study explored how restricting the amino acid diversity in ancestral enzymes can uncover alternative catalytic functions, including ATP formation from ADP Overall, the study shows that restricting amino acid diversity can reveal catalytic capabilities beyond a protein’s conventional function. By…
These monooxygenases depend on partner proteins that shuttle electrons from nicotinamide cofactors into the P450 active site. The structural comparison produced a surprise. Although the M. maydis enzyme, UmaCPR, shares greater sequence similarity with ascomycete CPRs, which include the Candida enzyme, its…
Chemists and enzymologists have long relied on mechanism-guided design to create new enzymatic reactions, but a team writing in Nature Catalysis reports a complementary strategy: systematically screening enzyme–photocatalyst pairings to let unexpected reactivity surface on its own. Among the…
Aug 01, 2024, 05:00 ET Research on ferroptosis is gaining momentum, but the development of small molecule inhibitors faces numerous challenges BEIJING, Aug. 1, 2024 /PRNewswire/ -- Glutathione peroxidase 4 (GPX4) is recognized as a critical regulator of ferroptosis, playing a significant role in lipid and amino acid…
A team of researchers has uncovered how subtle structural changes in terpene synthases can dramatically improve the biosynthesis of miltiradiene, a critical intermediate in the production of pharmacologically valuable compounds such as tanshinone IIA, triptolide, and carnosic acid. Boosting Production…
A newly synthesized thioxoacetamide compound shows sulfur-driven hydrogen bonding in its crystal structure and favorable predicted binding to human thymidine kinase 1 in computational models.
Cryo-EM and functional assays show that the JN.4-specific A475V substitution remodels the SARS-CoV-2 RBD large loop and reduces ACE2-binding affinity 3.5-fold compared with BA.2.86. Hydrophobic loop remodeling weakens ACE2 binding in SARS-CoV-2 JN.4 Subject of Research: Structural and functional effects of…
Engineers boosted a GH5 endoglucanase's catalytic efficiency up to 2.73-fold by mutating solvent-exposed peripheral residues to loosen the catalytic cavity, though the gains came with reduced thermal stability. Subject of Research: Protein engineering of the peripheral regions of a GH5 endoglucanase to…
Single-Molecule FRET Kinetics of the Mn2+ Riboswitch: Evidence for Allosteric Mg2+ Control of “Induced-Fit” vs “Conformational Selection” Folding Pathways H.-L.. Sung and D. J. Nesbitt, The Journal... Other ways to search: Nesbitt Group - Single Molecule Biophysics: Microscopy, Kinetics and Thermodynamics
POSTECH announced on the 20th that a research team led by Professor Seung Hwan Cho in the Department of Chemistry has developed a synthetic method that creates two side-by-side stereocenters within a single molecule in one reaction step and achieves the…
Seeing biomolecules atom by atom: cryo-electron microscopy, X-ray crystallography, structures of molecular machines and AI structure prediction.
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