Confirming that the enzyme had indeed bound to the support required structural evidence. Together, these techniques validated the successful immobilization, yielding a preparation the researchers designate LX_LadA. The performance gains reported for the immobilized enzyme are substantial. It also sustained high…
Researchers report a convenient synthesis of carboxypolyamine substrates and hydroxylamine-containing analogs designed to selectively inhibit carboxyspermidine decarboxylase in bacterial polyamine biosynthesis. Biogenic polyamines such as spermidine and spermine are small organic polycations that are vitally important across all domains of…
Researchers constructed a fused-grid template model of the human enzyme CYP1B1 and, paired with an existing CYP1A1 template, verified more than 260 metabolism and inhibition reactions with reported accuracy above 99 percent. Subject of Research: An in silico fused-grid template system for predicting CYP1A1- and…
Chemists report two simplified routes to trifluoromethylpyrazoles, including a one-hour Aliquat 336-catalyzed coupling that avoids major side products.
Combinatorial biosynthesis in Magnaporthe grisea shows that cytochalasan P450 enzymes tolerate macrocycle size differences but are restricted by the stereochemistry of methyl groups around the backbone.
Wollongong-based online news outlet covering Australia and beyond. If you have anything newsworthy - a story, video or picture to share with us, please email us. Enzymes are the molecular tools of life. They accelerate chemical reactions and are able to…
Study explored how restricting the amino acid diversity in ancestral enzymes can uncover alternative catalytic functions, including ATP formation from ADP Overall, the study shows that restricting amino acid diversity can reveal catalytic capabilities beyond a protein’s conventional function. By…
For much of human history, animals and plants were perceived to follow a different set of rules than the rest of the universe. In the 18th and 19th centuries, this culminated in a belief that living organisms were infused by a non-physical…
A new Frontiers in Chemistry review surveys ruthenium-catalyzed C–H activation, annulation, and hydrogen-borrowing strategies for building bioactive heterocycles. Ruthenium Catalysis Emerges as a Versatile Route to Bioactive Heterocycles
These monooxygenases depend on partner proteins that shuttle electrons from nicotinamide cofactors into the P450 active site. The structural comparison produced a surprise. Although the M. maydis enzyme, UmaCPR, shares greater sequence similarity with ascomycete CPRs, which include the Candida enzyme, its…
Enzymes as catalysts for making chemicals: enzyme engineering for synthesis, directed evolution and biocatalytic manufacturing routes.
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